Human Eg5 ELISA Kit
Sensitivity
0.05 ng/mL
Range
1.56-100 ng/mL
Reactivity
Human
Cat no : KE00893
Synonyms
KIF11, Kinesin-like protein 1, Kinesin-like protein KIF11, Kinesin-like spindle protein HKSP, Kinesin-related motor protein Eg5
Validation Data Gallery
Product Information
KE00893 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Eg5 ELISA kit is to be used to detect and quantify protein levels of endogenous human Eg5. The assay recognizes human Eg5. An antibody specific for human Eg5 has been pre-coated onto the microwells. The human Eg5 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Eg5 is added to detect the captured human Eg5 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Eg5 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.05 ng/mL |
| Range | 1.56-100 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3832 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 93% | 88%-103% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 50.68 | 2.91 | 5.74 |
| 2 | 8 | 11.37 | 0.30 | 2.67 |
| 3 | 8 | 6.12 | 0.24 | 3.98 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 51.11 | 3.07 | 6.00 |
| 2 | 16 | 10.89 | 0.57 | 5.27 |
| 3 | 16 | 5.94 | 0.31 | 5.20 |
Background Information
Eg5 is a plus-end-directed microtubule motor encoded by KIF11 gene. It is an essential mitotic kinesin that plays a pivotal role in the formation and maintenance of bipolar spindle during early prometaphase. Eg5 has been implicated in tumourigenesis, and its overexpression has been found in various cancer tissues.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | KIF11, Kinesin-like protein 1, Kinesin-like protein KIF11, Kinesin-like spindle protein HKSP, Kinesin-related motor protein Eg5 |