Recombinant Human EMR1/ADGRE1/(F4/80) protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg1716
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <1.0 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human EMR1/ADGRE1/(F4/80) protein His21-Asp599 (Accession# Q14246-1) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 2015 |
| Accession | Q14246-1 |
| PredictedSize | 89.4 kDa |
| SDS-PAGE | |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
EMR1 (EGF-like module containing mucin-like hormone receptor 1), also known as Adhesion G protein-coupled receptor E1 (ADGRE1), is a surface receptor with seven transmembrane segments that belong to the EGF-7-transmembrane family of G protein-coupled receptors. EMR1 expression is restricted to eosinophilic granulocytes, where expression is overlapping with the eotaxin receptor CCR3 and the immunoglobulin-like lectin Siglec-8. Absence on other leukocytes, including basophils, implies that EMR1 is a highly specific marker for eosinophils in humans and may be used as a novel therapeutic target for eosinophilic disorders. F4/80, the murine homolog of EMR1, is a marker of murine macrophage.
References:
1. Kwakkenbos, Mark J et al. Immunogenetics vol. 55,10 (2004): 655-66. 2. Baud, V et al. Genomics vol. 26,2 (1995): 334-44. 3. Hamann, Jörg et al. European journal of immunology vol. 37,10 (2007): 2797-802. 4. Legrand, Fanny et al. The Journal of allergy and clinical immunology vol. 133,5 (2014): 1439-47, 1447.e1-8. 5. Austyn, J M, and S Gordon. European journal of immunology vol. 11,10 (1981): 805-15.