Human DOPA decarboxylase/DDC ELISA Kit
Sensitivity
0.03 ng/mL
Range
0.156-10 ng/mL
Reactivity
Human
Cat no : KE00945
Synonyms
DDC, DOPA decarboxylase, AADC, Aromatic-L-amino-acid decarboxylase, EC:4.1.1.28
Validation Data Gallery
Product Information
KE00945 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human DOPA decarboxylase/DDC ELISA kit is to be used to detect and quantify protein levels of endogenous human DOPA decarboxylase/DDC. The assay recognizes human DOPA decarboxylase/DDC. An antibody specific for human DOPA decarboxylase/DDC has been pre-coated onto the microwells. The human DOPA decarboxylase/DDC protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human DOPA decarboxylase/DDC is added to detect the captured human DOPA decarboxylase/DDC protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human DOPA decarboxylase/DDC ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.03 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1644 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 71% | 70%-72% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 4.23 | 0.15 | 3.63 |
| 2 | 8 | 1.04 | 0.02 | 1.60 |
| 3 | 8 | 0.61 | 0.02 | 3.34 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 4.11 | 0.25 | 6.11 |
| 2 | 16 | 1.06 | 0.04 | 4.04 |
| 3 | 16 | 0.59 | 0.03 | 4.46 |
Background Information
DOPA decarboxylase (DDC), also known as aromatic l-amino acid decarboxylase, belongs to the pyridoxal-dependent aminotransferase superfamily. DDC is an enzyme that converts levodopa into dopamine, the latter being severely depleted in LBD due to the loss of dopaminergic neurons in the substantia nigra. DDC plays an important role in the dopaminergic system and participates in the uptake and decarboxylation of amine precursors in the peripheral tissues. It is reported that DDC may be a novel and potential marker for the detection of peritoneal micrometastases of gastric cancer.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | DDC, DOPA decarboxylase, AADC, Aromatic-L-amino-acid decarboxylase, EC:4.1.1.28 |