Human DNA2 ELISA Kit
Sensitivity
52.6 pg/mL
Range
78.1-5000 pg/mL
Reactivity
Human
Cat no : KE00654
Synonyms
DNA2L, KIAA0083
Validation Data Gallery
Product Information
KE00654 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human DNA2 ELISA kit is to be used to detect and quantify protein levels of endogenous human DNA2. The assay recognizes human DNA2. An antibody specific for human DNA2 has been pre-coated onto the microwells. The human DNA2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human DNA2 is added to detect the captured human DNA2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human DNA2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 52.6 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1763 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 94% | 81%-111% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,636.3 | 224.0 | 8.5 |
| 2 | 8 | 564.0 | 21.4 | 3.8 |
| 3 | 8 | 270.0 | 17.2 | 6.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,741.0 | 263.5 | 9.6 |
| 2 | 16 | 558.6 | 26.6 | 4.8 |
| 3 | 16 | 274.6 | 15.2 | 5.5 |
Background Information
DNA2(DNA replication ATP-dependent helicase/nuclease DNA2) is also named as DNA2L, KIAA0083 and belongs to the DNA2/NAM7 helicase family, plays versatile roles in processing DNA intermediates during DNA replication and repair. This protein includes 2 domains: DNA replication nuclease and DNA replication ATP-dependent helicase. It is a conserved helicase/nuclease involved in the maintenance of mitochondrial and nuclear DNA stability and it can interact with DNA polymerase gamma and stimulating its activity. It can also process intermediate 5' flap structures occuring in DNA replication long-patch base excision repair in mitochondria. DNA2 expression was significantly increased in human cancers and its expression correlated with patient outcome.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | DNA2L, KIAA0083 |