Recombinant Human DKK1 protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg3114
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human DKK1 protein Thr32-His266 (Accession# O94907) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 22943 |
| Accession | O94907 |
| PredictedSize | 51.4 kDa |
| SDS-PAGE | 58-75 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
DKK1 is a member of the DKK protein family. Other members of this family include DKK2, DKK3, and DKK4. The DKK family proteins share 35-40 % homology between various members. DKK1 is a secreted protein that functions as a negative regulator of Wnt/β-catenin signaling pathway. Wnt/ β-catenin signaling pathway involves binding of the Wnt ligands to the seven-transmembrane receptor, Frizzled, and the coreceptor lipoprotein-related protein 5 and 6 (LRP5/6). DKK1 forms a ternary complex with LRP5/6 and another receptor, Kremen, followed by endocytosis of this complex and removal of LRP5/6 from the cell surface. It has been suggested that DKK1 permits anterior development by inhibiting Wnt/ β-catenin signaling, which is essential for posterior patterning in vertebrates. The balance between Wnt signaling and DKK1 inhibition is critical for bone formation and homeostasis and insufficient or excess DKK1 activity in bone results in increased or decreased bone density, respectively.
References:
1. Niehrs, C. Oncogene vol. 25,57 (2006): 7469-81. 2. Mao, Bingyu et al. Nature vol. 417,6889 (2002): 664-7. 3. Krupnik, V E et al. Gene vol. 238,2 (1999): 301-13.