Speedy™ Human Complement factor D One-Step ELISA Kit
Sensitivity
2.2 pg/mL
Range
7.8-500 pg/mL
Reactivity
Human
Cat no : SE50078
Synonyms
CFD, ADIPSIN, C3 convertase activator, complement factor D (adipsin), DF
Validation Data Gallery
Product Information
| Product name | Speedy™ Human Complement factor D One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine |
| Assay type | Sandwich |
| Sensitivity | 2.2 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1675 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 89% | 84%-94% |
| Cell culture supernatant | 97% | 85%-112% |
| Urine | 101% | 88%-115% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 268.6 | 4.6 | 1.7 |
| 2 | 8 | 68.3 | 2.9 | 4.2 |
| 3 | 8 | 32.7 | 1.6 | 4.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 275.1 | 9.0 | 3.3 |
| 2 | 16 | 68.0 | 2.8 | 4.1 |
| 3 | 16 | 32.2 | 1.5 | 4.7 |
Background Information
Complement factor D, also known as adipsin, is a highly specific serine protease. Complement factor D is the rate-limiting enzyme in the alternative pathway of complement. It activates C3 convertase by cleaving factor B, which is a key step in the alternative pathway. Complement factor D catalyzes the cleavage of factor B to generate Ba (non-catalytic chain) and Bb (active catalytic subunit), which together with C3(H2O) (or C3b) constitute the active C3 convertase C3(H2O)Bb (or C3bBb).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CFD, ADIPSIN, C3 convertase activator, complement factor D (adipsin), DF |