Human Clusterin ELISA Kit0 Publications

カタログ番号: KE00110

51,000円

(希望販売価格)

容量: 1 Kit (96 assays)

販売および在庫状況はコスモ・バイオ(株)にお問い合わせください。
distributor

Images See all

概要


製品名:
Human Clusterin ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatants, Urine, Saliva

アッセイ方法:
Sandwich

感度:
5.0 pg/mL

測定範囲:
62.5-4000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Human serum 102% 91%-118%
Cell culture supernatants 96% 72%-121%
Urine 99% 85%-123%
Saliva 93% 80%-112%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 1,832.2 52.3 2.9
2 20 451.4 12.3 2.7
3 20 109.4 5.0 4.6

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 1,771.9 96.8 5.5
2 24 427.4 22.9 5.3
3 24 95.8 8.2 8.5

製品概要
KE00110 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Clusterin ELISA kit is to be used to detect and quantify protein levels of endogenous Clusterin. The assay recognizes human Clusterin. An antibody specific for Clusterin has been pre-coated onto the microwells. The Clusterin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human Clusterin is added to detect the captured human Clusterin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
AAG4, Apo J, APOJ, ApoJalpha, ApoJbeta, Apolipoprotein J, CLI, CLU, clusterin, Ku70 binding protein 1, KUB1, NA1/NA2, SGP 2, SGP2, SP 40, TRPM 2, TRPM2
背景説明

Clusterin (also known as apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biologic fluids. It is encoded by one single gene and cleaved posttranslationally into α and β subunits prior to secretion from the cell. Many functions have been described for clusterin, including regulating apoptosis, transporting lipids, controlling cell interactions, regulating complement. Increased plasma clusterin levels have been associated with severity, pathology, and progression in Alzheimer disease.


Back
to top