Recombinant Human CHI3L1/YKL40 protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg4992
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human CHI3L1 protein Tyr22-Thr383 (Accession# P36222) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 1116 |
| Accession | P36222 |
| PredictedSize | 66.5 kDa |
| SDS-PAGE | 60-70 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
CHI3L1 (Chitinase-3-Like Protein 11), also known as YKL-40 or HC-gp39, is a secreted glycoprotein and a member of the chitinase-like protein family. Although it lacks chitinase activity, it possesses carbohydrate-binding capacity and participates in inflammatory responses, tissue remodeling, and immune regulation. At the mechanistic level, CHI3L1 can activate multiple signaling pathways such as STAT3, MAPK, and PI3K/Akt, and bind to various receptors including IL-13Rα2 and CD44, thereby promoting cell survival and resistance to apoptosis.
References:
1. Song, Yanna et al. Cell reports vol. 43,5 (2024): 114226. 2. Lananna, Brian V et al. Science translational medicine vol. 12,574 (2020): eaax3519. 3. Yu, Wanli et al. Developmental cell vol. 60,12 (2025): 1751-1767.e9.