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Recombinant Human CXCL2 protein (rFc Tag) (HPLC verified)

Species

Human

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg3423

Validation Data Gallery

  • Purity of Recombinant Human CXCL2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human CXCL2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Human CXCL2 was greater than 90% as determined by SEC-HPLC.

    The purity of Human CXCL2 was greater than 90% as determined by SEC-HPLC.

Product Information

Purity >90 %, SDS-PAGE
>90 %, SEC-HPLC
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human CXCL2 protein Ala35-Asn107 (Accession# P19875) with a rabbit IgG Fc tag at the N-terminus.
GeneID 2920
Accession P19875
PredictedSize 35.1 kDa
SDS-PAGE 33-40 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Chemokine (C-X-C motif) ligand 2 (CXCL2) is a small cytokine belonging to the CXC chemokine family that is also called macrophage inflammatory protein 2-alpha (MIP2-alpha), Growth-regulated protein beta (Gro-beta) and Gro oncogene-2 (Gro-2). This chemokine is secreted by monocytes and macrophages and is chemotactic for polymorphonuclear leukocytes and hematopoietic stem cells.  CXCL2 mobilizes cells by interacting with a cell surface chemokine receptor called CXCR2. During inflammation, the level of CXCL2 increases and is involved in the release of opioid peptides from leukocytes. Down-regulation of CXCL2 could reduce the recruitment of polymorphonuclear cells, which are key players in the clearance of L. monocytogenes during infection.

References:

1. Wolpe SD, et al. (1989). PNAS. 86(2):612-6.
2. Iida N, et al. (1990). Mol Cell Biol. 10(10):5596-9.
3. Pelus LM, et al. (2006). Exp Hematol. 34(8):1010-20.
4. Dagmar Hackel, et al (2010). NeuroImmune Biology. 9,237-250.
5. Febbraio MA. et al. (2005). Exerc Sport Sci Rev. 33:114-9.
6. L. Dortet, et al. (2019). Encyclopedia ofMicrobiology. 803-818.
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