Recombinant Human CXCL16 protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg2165
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <1.0 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human CXCL16 protein Asn30-Thr205 (Accession# Q9H2A7) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 58191 |
| Accession | Q9H2A7 |
| PredictedSize | 45.4 kDa |
| SDS-PAGE | |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
CXCL16 was discovered as a ligand for CXC chemokine receptor (CXCR) 6. CXCL16 is a membrane-bound chemokine that consists of four distinct domains: the chemokine domain, mucin-like domain, transmembrane domain and ad cytoplasmic. After cleavage, soluble CXCL16 acts as a chemoattractant for activated CD8 T cells, NKT cells and Th1-polarized T cells that express CXCR6. CXCL16 expression has been studied mainly in macrophages and dendritic cells. CXCL16 production by non-immune cells remains controversial. CXCL16 was expressed as two different forms: a 50 kDa form and a 28 kDa form, and the 50 kDa may be the glycosylated form.
References:
1. Tohyama, Mikiko et al. International immunology vol. 19,9 (2007): 1095-102. 2. Abu El-Asrar, Ahmed M et al. Frontiers in immunology vol. 11 (2021):60163. 3. Tohyama, Mikiko et al. International immunology vol. 19,9 (2007): 1095-102.