Human CXCL16 ELISA Kit1 Publications

カタログ番号: KE00052

51,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human CXCL16 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatants

アッセイ方法:
Sandwich

感度:
0.1 pg/mL

測定範囲:
7.8-500 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Human serum 91% 76%-103%
Human plasma 99% 83%-119%
Cell culture supernatants 87% 82%-118%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 241.1 10.8 4.5
2 20 56.1 1.7 3.0
3 20 12.7 0.5 3.9

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 260.7 17.1 6.6
2 24 56.0 3.2 5.8
3 24 12.7 0.7 5.8

製品概要
KE00052 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CXCL16 ELISA kit is to be used to detect and quantify protein levels of endogenous CXCL16. The assay recognizes human CXCL16. An antibody specific for CXCL16 has been pre-coated onto the microwells. The CXCL16 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for CXCL16 is added to detect the captured CXCL16 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
C X C motif chemokine 16, CXCL16, CXCLG16, SCYB16, Small inducible cytokine B16, SR PSOX, SRPSOX, Transmembrane chemokine CXCL16
背景説明

CXC chemokine ligand 16 (CXCL16) is a CXC soluble chemokine, an adhesion molecule and a cell surface scavenger receptor. CXCL16 regulates inflammation, tissue injury and fibrosis. CXCL16 exists as a transmembrane form (TM-CXCL16) as well as a soluble form (sCXCL16) that is cleaved by proteolytic enzymes. CXCL16 is produced by several inflammatory cells including macrophages, dendritic cells, smooth muscle cells or lymphocytes. CXCL16 is expressed in various cancers, including pancreatic, prostate, breast, colorectal, and nonsmall cell lung cancer. CXCL16) has been proposed as an important pathogenic mediator in inflammatory diseases, such as rheumatoid arthritis, hepatitis or atherosclerosis.


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