Human CXCL10/IP-10 ELISA Kit
Cat no : KE00128
Synonyms
CXCL10, CXCL10/IP10, IP10, 10 kDa interferon gamma-induced protein, C X C motif chemokine 10
Validation Data Gallery
Product Information
KE00128 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CXCL10 ELISA kit is to be used to detect and quantify protein levels of endogenous CXCL10. The assay recognizes human CXCL10. An antibody specific for CXCL10 has been pre-coated onto the microwells. The CXCL10 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for CXCL10 is added to detect the captured CXCL10 protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CXCL10/IP-10 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Saliva |
| Assay type | Sandwich |
| Sensitivity | 1.2 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3627 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 94% | 83%-103% |
| Human saliva | 99% | 89%-112% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 583.5 | 28.9 | 5.0 |
| 2 | 8 | 144.1 | 5.5 | 3.8 |
| 3 | 8 | 43.1 | 1.1 | 2.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 553.1 | 43.7 | 7.9 |
| 2 | 16 | 141.9 | 6.1 | 4.3 |
| 3 | 16 | 42.1 | 2.1 | 5.1 |
Background Information
CXCL10 ( also known as IP-10) is a member of the CXC chemokine family which binds to the CXCR3 receptor to exert its biological effects. CXCL10 is a 12-kDa protein and constitutes two internal disulfide cross bridges. The predicted signal peptidase cleavage generates a 10-kDa secreted polypeptide with four conserved cysteine residues in the N-terminal. The CXCL10 gene localizes on chromosome 4 at band q21, a locus associated with an acute monocytic/B-lymphocyte lineage leukemia exhibiting translocation of t (4; 11) (q21; q23). CXCL10 mediates leukocyte trafficking, adaptive immunity, inflammation, haematopoiesis and angiogenesis. Under proinflammatory conditions CXCL10 is secreted from a variety of cells, such as leukocytes, activated neutrophils, eosinophils, monocytes, epithelial cells, endothelial cells, stromal cells (fibroblasts) and keratinocytes in response to IFN-γ.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CXCL10, CXCL10/IP10, IP10, 10 kDa interferon gamma-induced protein, C X C motif chemokine 10 |
Publications
| Species | Sample Type | Title |
|---|---|---|
Gastroenterology A-to-I RNA-Editing: an epigenetic hallmark cannot be ignored in silencing the tumor microenvironment and is promising in predicting immunotherapy response for esophageal squamous cell carcinoma | ||
Drug Resist Updat Stabilin-1(+) lipid-associated macrophages promote lung adenocarcinoma liver metastasis and osimertinib resistance through impairing macrophage phagocytosis via SIRPα-CD47 axis. | ||
Adv Sci (Weinh) S100A5 Attenuates Efficiency of Anti-PD-L1/PD-1 Immunotherapy by Inhibiting CD8+ T Cell-Mediated Anti-Cancer Immunity in Bladder Carcinoma | ||
Adv Sci (Weinh) BCAT2 Shapes a Noninflamed Tumor Microenvironment and Induces Resistance to Anti-PD-1/PD-L1 Immunotherapy by Negatively Regulating Proinflammatory Chemokines and Anticancer Immunity | ||
Cell Death Differ O-GlcNAcylation of UGDH regulates its activity and remodels the extracellular matrix to facilitate tumor growth. | ||
J Immunother Cancer Overcoming immunotherapy resistance in bladder cancer with a novel antibody-drug conjugate RC48 |