Human CLPTM1 ELISA Kit
Sensitivity
0.11 ng/mL
Range
0.47-30 ng/mL
Reactivity
Human
Cat no : KE00602
Synonyms
Putative lipid scramblase CLPTM1
Validation Data Gallery
Product Information
KE00602 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CLPTM1 ELISA kit is to be used to detect and quantify protein levels of endogenous human CLPTM1. The assay recognizes human CLPTM1. An antibody specific for human CLPTM1 has been pre-coated onto the microwells. The human CLPTM1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CLPTM1 is added to detect the captured human CLPTM1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CLPTM1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.11 ng/mL |
| Range | 0.47-30 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1209 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 91% | 84%-100% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 15.01 | 0.43 | 2.87 |
| 2 | 8 | 3.82 | 0.29 | 7.70 |
| 3 | 8 | 2.09 | 0.10 | 4.96 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 14.51 | 0.95 | 6.52 |
| 2 | 16 | 3.79 | 0.25 | 6.70 |
| 3 | 16 | 2.01 | 0.13 | 6.35 |
Background Information
Cleft lip and palate transmembrane protein 1 (CLPTM1), a multipass transmembrane protein, was identified due to the association of a chromosomal translocation with a cleft lip and palate. CLPTM1 regulates epileptic seizures by modulating GABAAR-mediated inhibitory synaptic transmission. Highly expressed CLPTM1L may contribute to a poor prognosis and increase invasion, proliferation and migration of oral squamous cell carcinoma.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Putative lipid scramblase CLPTM1 |