Human CEBP Alpha/CEBPA ELISA Kit
Sensitivity
0.02 ng/mL
Range
0.625-40 ng/mL
Reactivity
Human
Cat no : KE00873
Synonyms
CEBP alpha, CEBPA, C/EBP alpha, C/EBPα, CCAAT/enhancer-binding protein alpha
Validation Data Gallery
Product Information
KE00873 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CEBP Alpha/CEBPA ELISA kit is to be used to detect and quantify protein levels of endogenous human CEBP Alpha/CEBPA. The assay recognizes human CEBP Alpha/CEBPA. An antibody specific for human CEBP Alpha/CEBPA has been pre-coated onto the microwells. The human CEBP Alpha/CEBPA protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CEBP Alpha/CEBPA is added to detect the captured human CEBP Alpha/CEBPA protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CEBP Alpha/CEBPA ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.625-40 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1050 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 82% | 71%-88% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 21.20 | 1.04 | 4.92 |
| 2 | 8 | 5.51 | 0.27 | 4.92 |
| 3 | 8 | 2.85 | 0.15 | 5.36 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 21.87 | 1.15 | 5.27 |
| 2 | 16 | 5.40 | 0.22 | 4.13 |
| 3 | 16 | 2.97 | 0.17 | 5.75 |
Background Information
CEBPA and its isoforms play important roles in lineage determination and gene activation in a variety of cell types by activating transcription from lineage-specific promoters. CEBPA is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. In hematopoiesis, C/EBPa is a key factor in driving the development of myeloid cells interacting with a variety of factors, including c-Myc, PU.1, and microRNAs. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin which plays an important role in body weight homeostasis. CEBPA can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. Several pathways have been implicated as the means by which CEBPA mediates cell cycle arrest and proliferation, including p21, cyclin-dependent kinases and the E2F complex via c-Myc.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CEBP alpha, CEBPA, C/EBP alpha, C/EBPα, CCAAT/enhancer-binding protein alpha |