Human CDK9 ELISA Kit
Sensitivity
4.4 pg/mL
Range
31.25-2000 pg/mL
Reactivity
Human
Cat no : KE00641
Synonyms
C 2k, C-2K, CDC2L4, Cell division cycle 2-like protein kinase 4, CTK1
Validation Data Gallery
Product Information
KE00641 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CDK9 ELISA kit is to be used to detect and quantify protein levels of endogenous human CDK9. The assay recognizes human CDK9. An antibody specific for human CDK9 has been pre-coated onto the microwells. The human CDK9 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CDK9 is added to detect the captured human CDK9 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CDK9 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 4.4 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1025 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 110% | 104%-119% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,151.6 | 30.2 | 2.6 |
| 2 | 8 | 291.0 | 13.4 | 4.6 |
| 3 | 8 | 154.0 | 4.2 | 2.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,127.2 | 43.8 | 3.9 |
| 2 | 16 | 275.9 | 21.3 | 7.7 |
| 3 | 16 | 149.8 | 6.3 | 4.2 |
Background Information
CDK9 (Cyclin-dependent kinase 9) is a member of the Cdc2-like family of kinases. Its cyclin partners are members of the family of cyclin T (T1, T2a and T2b) and cyclin K. The CDK9/cyclin T complexes appear to be involved in regulating several physiological processes. CDK9 has also been described as the kinase of the TAK complex, which is homologous to the P-TEFb complex and involved in HIV replication. In addition, CDK9 seems to have an anti-apoptotic function in monocytes, that may be related to its control over differentiation of monocytes. CDK9 is highly expressed in prostate cancer, particularly tumors with aggressive phenotypes, reinforcing its potential relevance as a therapeutic target.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | C 2k, C-2K, CDC2L4, Cell division cycle 2-like protein kinase 4, CTK1 |