Speedy™ Human CD80/B7-1 One-Step ELISA Kit
Sensitivity
28.7 pg/mL
Range
78.1-5000 pg/mL
Reactivity
Human
Cat no : SE50185
Synonyms
CD80, Activation B7 1 antigen, Activation B7-1 antigen, B7, B7 1
Validation Data Gallery
Product Information
| Product name | Speedy™ Human CD80/B7-1 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 28.7 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 941 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 79% | 71%-85% |
| Cell culture supernatant | 106% | 100%-112% |
| Urine | 97% | 87%-105% |
| Cell lysate | 76% | 71%-82% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,458.7 | 72.2 | 2.9 |
| 2 | 8 | 583.6 | 15.1 | 2.6 |
| 3 | 8 | 281.4 | 7.0 | 2.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,581.8 | 141.5 | 5.5 |
| 2 | 16 | 612.8 | 33.9 | 5.5 |
| 3 | 16 | 288.4 | 10.7 | 3.7 |
Background Information
CD80, also known as B7-1, is a type I membrane protein and a member of the immunoglobulin superfamily. It plays a crucial role in T cell activation and regulation by providing a co-stimulatory signal necessary for T cell proliferation and cytokine production. CD80 interacts with CD28 on T cells to promote T cell activation and with CTLA-4 to inhibit T cell activation. This dual role highlights its importance in modulating immune responses. Additionally, CD80 acts as a receptor for adenovirus subgroup B, indicating its involvement in viral infections.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD80, Activation B7 1 antigen, Activation B7-1 antigen, B7, B7 1 |