Product Information
CD8 is a cell surface glycoprotein found on most cytotoxic T lymphocytes and is used as a marker for these cells. It acts as a coreceptor for T cell receptor and functions to recognize MHC –I antigens. 10%-30% human lymphocytes are CD8 positive. Human CD8 Magnetic Beads Kit is used for isolation or depletion of human CD8 T lymphocytes from PBMC, whole blood, or other sample types. Following incubation with biotinylated human CD8 antibody and streptavidin conjugated magnetic beads, the cell sample is placed on a magnet. CD8+ cells remain attached to magnetic beads after separation and can be used for downstream applications, such as cell expansion, but are not suitable for flow cytometry analysis. CD8- cells remain in the supernatant and can be used for further applications.
Components
KMS003-10: · MS001-10: 100µL 10mg/mL streptavidin magnetic beads · MS65204-10: 100 µL 0.1mg/mL Biotin-CD8 (clone: UCHT4) KMS003-100: · MS001-100: 1mL 10mg/mL streptavidin magnetic beads · MS65204-100: 1mL 0.1mg/mL Biotin-CD8 (clone: UCHT4) |
Storage
2-8°C
Storage buffer
Streptavidin beads: PBS, pH7.4, 0.2% BSA and 0.05% Sodium Azide
Biotin Antibody: PBS, pH7.4, 0.2% BSA and 0.09% Sodium Azide
Reactivity
Human
Recommend usage
10 µL Biotin-CD8 antibody and 10 µL streptavidin beads for 1*107 cells
Cited in Article as
kms003, Human CD8 Magnetic Beads Kit, Proteintech, IL, USA
Documentation
| Datasheet |
|---|
| Human CD8 Magnetic Beads Kit KMS003 datasheet |