Human CD47 ELISA Kit
Sensitivity
5.5 pg/mL
Range
78.1-5000 pg/mL
Reactivity
Human
Cat no : KE00499
Synonyms
CD47,CD47 molecule,IAP,Integrin associated protein
Validation Data Gallery
Product Information
KE00499 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CD47 ELISA kit is to be used to detect and quantify protein levels of endogenous human CD47. The assay recognizes human CD47. An antibody specific for human CD47 has been pre-coated onto the microwells. The human CD47 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CD47 is added to detect the captured human CD47 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CD47 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 5.5 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 961 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 107% | 101%-114% |
| Tissue lysate | 76% | 72%-80% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,189.8 | 112.5 | 5.1 |
| 2 | 8 | 579.8 | 38.4 | 6.6 |
| 3 | 8 | 278.5 | 12.6 | 4.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,269.9 | 146.8 | 6.5 |
| 2 | 16 | 569.7 | 35.1 | 6.2 |
| 3 | 16 | 285.1 | 15.2 | 5.3 |
Background Information
CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts as a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD47,CD47 molecule,IAP,Integrin associated protein |