Product Information
Memory T cells are antigen-exposed T lymphocytes which would mediate fast and robust response upon repeat encounter with antigen. Human CD4 Memory T Cell Isolation kit is designed to isolate untouched CD4+ memory T lymphocytes which contains a biotin labeled antibody cocktail and streptavidin conjugated magnetic beads. Through incubation of samples with biotin antibody cocktail followed by incubation of streptavidin beads, the magnetically labeled non CD4+ memory cells will be retained in the tube by magnetic separator. The untouched CD4+ T memory cells are collected in supernatant. Any downstream application can be performed on CD4+ memory cells after separation, such as Flow Cytometry and in vitro culture.
Components
KMS305-10:
·MS305A-10: 300 µL Human CD4 Isolation Biotin Antibody Cocktail (CD8, CD19, CD14, CD56, CD45RA, CD20, CD11b, CD16, CD36, CD235a, CD123, TCR γδ)
·MS305B-10: 300µL streptavidin magnetic beads
KMS305-100:
·MS305A-100: 3mL Human CD4 Isolation Biotin Antibody Cocktail (CD8, CD19, CD14, CD56, CD45RA, CD20, 11b, CD16, CD36, CD235a, CD123, TCR γδ)
·MS305B-100: 3mL streptavidin magnetic beads
Storage
2-8°C
Storage buffer
PBS, pH7.4, 0.2% BSA and 0.05% Sodium Azide
Reactivity
Human
Beads diameter
2.7 µm
Recommend usage
30µL biotin labeled antibody cocktail and 30µL streptavidin beads for 1x107 cells
Cited in Article as
kms305, Human CD4 Memory T Cell Isolation Kit, Proteintech, IL, USA
Documentation
| Datasheet |
|---|
| Human CD4 Memory T Cell Isolation Kit KMS305 datasheet |