Recombinant Human CD31 protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg4658
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human CD31 protein Gln28-Lys601 (Accession# P16284-1) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 5175 |
| Accession | P16284-1 |
| PredictedSize | 90.8 kDa |
| SDS-PAGE | 90-125 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. CD31 is also present on the surface of hematopoietic cells and immune cells, including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes, and some types of T-cells. In addition to its role in cell-cell adhesion, CD31 serves as a signaling receptor and is involved in crucial physiological events, including nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration, and inflammation and angiogenesis.
References:
1.Newman, P J. The Journal of clinical investigation vol. 99,1 (1997): 3-8. 2.McCormick, Margaret E et al. Arteriosclerosis, thrombosis, and vascular biology vol. 31,3 (2011): 643-9. 3.Ma, Liang et al. Proceedings of the National Academy of Sciences of the United States of America vol. 107,45 (2010): 19461-6. 4.Woodfin, Abigail et al. Arteriosclerosis, thrombosis, and vascular biology vol. 27,12 (2007): 2514-23. 5.Kim, Hyongbum et al. Circulation research vol. 107,5 (2010): 602-14.
