Speedy™ Human CD300A One-Step ELISA Kit
Sensitivity
6.7 pg/mL
Range
62.5-4000 pg/mL
Reactivity
Human
Cat no : SE50074
Synonyms
CD300 antigen-like family member A, CD300a molecule, CLM 8, CLM-8, CMRF 35 H9
Validation Data Gallery
Product Information
| Product name | Speedy™ Human CD300A One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Urine, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 6.7 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 11314 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 102% | 91%-112% |
| Urine | 109% | 107%-113% |
| Cell lysate | 81% | 77%-84% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,065.3 | 121.7 | 5.9 |
| 2 | 8 | 509.5 | 18.0 | 3.5 |
| 3 | 8 | 167.1 | 5.2 | 3.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,078.5 | 110.8 | 5.3 |
| 2 | 16 | 513.6 | 17.9 | 3.5 |
| 3 | 16 | 166.7 | 5.3 | 3.2 |
Background Information
CD300A is an inhibitory receptor expressed on various leukocytes. It plays a crucial role in modulating immune responses by recognizing phosphatidylserine (PS) and phosphatidylethanolamine (PE), two aminophospholipids exposed on the outer leaflet of dead and activated cells. This recognition helps regulate immune functions and the host's response to various disease states, such as infectious diseases, cancer, allergies, and chronic inflammatory diseases. CD300A delivers inhibitory signals through its immunoreceptor tyrosine-based inhibitory motifs (ITIMs), which are phosphorylated upon ligand binding and recruit SH2-containing phosphatases, leading to the inhibition of downstream signaling pathways.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD300 antigen-like family member A, CD300a molecule, CLM 8, CLM-8, CMRF 35 H9 |