Product Information
CD3 is a multimeric protein associated with T cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction. 45%-70% human peripheral blood mononuclear cells (PBMC) express CD3. Human CD3 Magnetic Beads Kit is used for isolation or depletion of human CD3 T lymphocytes from PBMC, whole blood, or other sample types. Following incubation with biotinylated human CD3 antibody and Streptavidin magnetic beads, the cell sample is placed on a magnet. CD3+ cells remain attached to magnetic beads after separation and can be used for downstream applications, such as in cell expansion. CD3- cells remain in supernatant and can also be used for further applications.
Components
KMS001-10:
· MS001-10: 100µL 10mg/mL streptavidin magnetic beads
· MS65133-10: 100µL 0.1mg/mL Biotin-CD3 (clone: OKT3)
KMS001-100:
· MS001-100: 1mL 10mg/mL streptavidin magnetic beads
· MS65133-100: 1mL 0.1mg/mL Biotin-CD3 (clone: OKT3)
Storage
2-8°C
Storage buffer
Streptavidin beads: PBS, pH7.4, 0.2% BSA and 0.05% Sodium Azide
Biotin Antibody: PBS, pH7.4, 0.2% BSA and 0.09% Sodium Azide
Reactivity
Human
Recommend usage
10µL Biotin-CD3 antibody and 10µL streptavidin beads for 1*107 cells
Cited in Article as
kms001, Human CD3 Magnetic Beads Kit, Proteintech, IL, USA
Documentation
| Datasheet |
|---|
| Human CD3 Magnetic Beads Kit KMS001 datasheet |
Publications
| Application | Title |
|---|---|
Immunol Med C5a stimulation induces caspase-1 activation and mature IL-1β production in human peripheral blood mononuclear cells |