Speedy™ Human CD25/IL-2RA One-Step ELISA Kit
Sensitivity
2.3 pg/mL
Range
31.25-2000 pg/mL
Reactivity
Human
Cat no : SE50037
Synonyms
CD25, IL 2 RA, IL 2 receptor subunit alpha, IL 2R subunit alpha, IL2 RA
Validation Data Gallery
Product Information
| Product name | Speedy™ Human CD25/IL-2RA One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 2.3 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3559 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 95% | 81%-112% |
| Cell culture supernatant | 107% | 95%-122% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,024.0 | 45.6 | 4.5 |
| 2 | 8 | 236.1 | 3.5 | 1.5 |
| 3 | 8 | 122.8 | 2.5 | 2.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,067.1 | 84.6 | 7.9 |
| 2 | 16 | 244.8 | 9.9 | 4.0 |
| 3 | 16 | 127.3 | 5.7 | 4.5 |
Background Information
CD25, also known as the α chain of the IL-2 receptor (IL-2Rα), is a key regulator of T cell function. It is expressed in activated T cells and has been implicated in regulating various immune and autoimmune responses. Its deficiency has been associated with autoimmune phenomena including autoimmune enteropathy and bullous pemphigoid. The soluble CD25 can be found in the serum and may have implications for human disease in both cancer and chronic infection.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD25, IL 2 RA, IL 2 receptor subunit alpha, IL 2R subunit alpha, IL2 RA |