Speedy™ Human CD23 One-Step ELISA Kit
Sensitivity
27.2 pg/mL
Range
62.5-4000 pg/mL
Reactivity
Human
Cat no : SE50087
Synonyms
BLAST 2, BLAST-2, CD23A, CLEC4J, C-type lectin domain family 4 member J
Validation Data Gallery
Product Information
| Product name | Speedy™ Human CD23 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 27.2 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2208 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 86% | 81%-92% |
| Cell culture supernatant | 102% | 94%-112% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,326.4 | 72.7 | 3.1 |
| 2 | 8 | 590.9 | 17.8 | 3.0 |
| 3 | 8 | 289.7 | 9.9 | 3.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,294.7 | 80.5 | 3.5 |
| 2 | 16 | 568.8 | 26.8 | 4.7 |
| 3 | 16 | 282.2 | 12.4 | 4.4 |
Background Information
CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells. CD23 can be cleaved into soluble fragment (soluble CD23, sCD23) of various sizes displaying pleiotropic biological activities. Increased plasma levels of sCD23 have been reported in allergic disorders, acute viral diseases, lepromatous leprosy, B-CLL, and inflammatory diseases like RA, SLE and sarcoidosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | BLAST 2, BLAST-2, CD23A, CLEC4J, C-type lectin domain family 4 member J |