Speedy™ Human CD22 One-Step ELISA Kit
Sensitivity
0.05 ng/mL
Range
0.156-10 ng/mL
Reactivity
Human
Cat no : SE50056
Synonyms
B cell receptor CD22, B-cell receptor CD22, BL-CAM, B-lymphocyte cell adhesion molecule, CD22 molecule
Validation Data Gallery
Product Information
| Product name | Speedy™ Human CD22 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.05 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 933 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 87% | 72%-117% |
| Cell lysate | 108% | 103%-117% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.32 | 0.09 | 1.69 |
| 2 | 8 | 1.32 | 0.02 | 1.52 |
| 3 | 8 | 0.66 | 0.01 | 1.52 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.30 | 0.07 | 1.32 |
| 2 | 16 | 1.32 | 0.02 | 1.52 |
| 3 | 16 | 0.66 | 0.01 | 1.52 |
Background Information
CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell. Soluble form of CD22 (sCD22) has been found in human serum and may be useful as a tumor marker for hairy cell leukemia.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | B cell receptor CD22, B-cell receptor CD22, BL-CAM, B-lymphocyte cell adhesion molecule, CD22 molecule |