Speedy™ Human CD14 One-Step ELISA Kit
Sensitivity
0.7 pg/mL
Range
15.6-1000 pg/mL
Reactivity
Human
Cat no : SE50054
Synonyms
CD14 molecule, Monocyte differentiation antigen CD14, membrane-bound form, Monocyte differentiation antigen CD14, urinary form, Myeloid cell-specific leucine-rich glycoprotein
Validation Data Gallery
Product Information
| Product name | Speedy™ Human CD14 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Saliva |
| Assay type | Sandwich |
| Sensitivity | 0.7 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 929 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 98% | 94%-103% |
| Saliva | 108% | 97%-122% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 526.7 | 10.4 | 2.0 |
| 2 | 8 | 134.6 | 2.1 | 1.6 |
| 3 | 8 | 41.6 | 0.6 | 1.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 531.1 | 12.6 | 2.4 |
| 2 | 16 | 135.9 | 2.5 | 1.8 |
| 3 | 16 | 42.0 | 1.2 | 2.9 |
Background Information
CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein. CD14 is preferentially expressed on monocytes and macrophages. CD14 acts as a co-receptor (along with TLR4 and MD-2) for bacterial liposaccharides (LPS). It plays a major role in the inflammatory response of monocytes to LPS. Soluble forms of CD14 (sCD14) have been detected in cell culture supernatants and in human serum and urine, either shedded from the cell surface or released from intracellular pools. Increased release of sCD14 from monocytes is observed after stimulation with various agents, and sCD14 may therefore be a marker for activation of monocytes/macrophages.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD14 molecule, Monocyte differentiation antigen CD14, membrane-bound form, Monocyte differentiation antigen CD14, urinary form, Myeloid cell-specific leucine-rich glycoprotein |