Speedy™ Human CD134/OX40 One-Step ELISA Kit
Sensitivity
10.9 pg/mL
Range
46.88-3000 pg/mL
Reactivity
Human
Cat no : SE50068
Synonyms
TNFRSF4, ACT35, ACT35 antigen, CD134, OX40
Validation Data Gallery
Product Information
| Product name | Speedy™ Human CD134/OX40 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 10.9 pg/mL |
| Range | 46.88-3000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7293 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 98% | 92%-102% |
| Cell culture supernatant | 106% | 97%-114% |
| Cell lysate | 80% | 75%-97% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,323.5 | 24.8 | 1.9 |
| 2 | 8 | 329.5 | 9.7 | 2.9 |
| 3 | 8 | 163.4 | 4.0 | 2.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,324.5 | 26.1 | 2.0 |
| 2 | 16 | 329.2 | 9.9 | 3.0 |
| 3 | 16 | 164.0 | 5.3 | 3.2 |
Background Information
OX40 (CD134) is a co-stimulatory receptor belonging to the TNF receptor family. It is expressed on activated CD4+ T cells and plays a crucial role in enhancing T cell proliferation, cytokine production, and survival. OX40 signaling leads to the activation of NF-κB and the upregulation of anti-apoptotic proteins such as Bcl-xL and Bcl-2. This receptor also promotes the differentiation of naive CD4+ T cells into high IL-4-producing effectors. OX40 ligand (OX40L) is expressed on antigen-presenting cells (APCs) such as activated B cells and dendritic cells. The interaction between OX40 and OX40L is critical for T cell activation and the regulation of immune responses.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | TNFRSF4, ACT35, ACT35 antigen, CD134, OX40 |