Human CD100 ELISA Kit
Sensitivity
1.1 pg/mL
Range
78.1-5000 pg/mL
Reactivity
Human
Cat no : KE00750
Synonyms
SEMA4D, A8, CD 100, CD100 antigen, SEM4D
Validation Data Gallery
Product Information
KE00750 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CD100 ELISA kit is to be used to detect and quantify protein levels of endogenous human CD100. The assay recognizes human CD100. An antibody specific for human CD100 has been pre-coated onto the microwells. The human CD100 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CD100 is added to detect the captured human CD100 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CD100 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 1.1 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 10507 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 109% | 93%-130% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,785.8 | 53.8 | 1.9 |
| 2 | 8 | 626.2 | 18.0 | 2.9 |
| 3 | 8 | 363.6 | 8.6 | 2.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,719.3 | 130.7 | 4.8 |
| 2 | 16 | 644.8 | 32.4 | 5.0 |
| 3 | 16 | 368.5 | 9.6 | 2.6 |
Background Information
CD100 (also known as SEMA4D), a 150 kDa homodimer, belongs to the family of immune semaphorins, and CD100 is abundantly expressed on resting T cells, NK cells, and antigen-presenting cells. As a transmembrane glycoprotein, it is digested to its soluble form, sCD100, by specific matrix metalloproteinases. CD100 has been reported that the expression levels of CD100, and one of its receptors, plexin-B1 (PLXNB1), are increased in head and neck, prostate, colon, breast, and lung cancers, and the interaction between them provides oncogenic signaling essential for tumor growth and metastasis. Elevated levels of CD100 have been observed in inflammatory and infectious diseases, although its exact role in these conditions is still under investigation. CD100 and its signaling pathways are increasingly being linked to various immunological diseases, making it a potential diagnostic marker and therapeutic target.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | SEMA4D, A8, CD 100, CD100 antigen, SEM4D |