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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
0
Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Purchase

Recombinant Human CCL27 protein (rFc Tag)(HPLC verified)

Species

Human

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg2461

Validation Data Gallery

  • Purity of Recombinant Human CCL27 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human CCL27 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Human CCL27 was greater than 90% as determined by SEC-HPLC.

    The purity of Human CCL27 was greater than 90% as determined by SEC-HPLC.

Product Information

Purity >90 %, SDS-PAGE
>90 %, SEC-HPLC
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human CCL27 protein Phe25-Gly112 (Accession# Q9Y4X3) with a rabbit IgG Fc tag at the N-terminus.
GeneID 10850
Accession Q9Y4X3
PredictedSize 37.3 kDa
SDS-PAGE 34-40 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CCL27 (also called ESkine, ALP, ILC, or CTACK) is a chemokine predominantly expressed by skin keratinocytes. In the healthy skin, CCL27 expression was predominantly detected in keratinocytes of the epidermis and hair follicles (HF) in both humans and mice. CCL27 expression is suppressed in the lesional skin of various skin inflammatory diseases such as psoriasis, hidradenitis suppurativa (HS), and alopecia areata .

References:

1.Davila, Micha L et al. (2022) iScience. 25(6):104426.
2.Zhong, Wenlong et al. (2020) Oncoimmunology. 9(1):1776060.