Human CCL24/Eotaxin-2/MPIF-2 ELISA Kit0 Publications

カタログ番号: KE00196

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human CCL24/Eotaxin-2/MPIF-2 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatants

アッセイ方法:
Sandwich

感度:
1.8 pg/mL

測定範囲:
7.8-500 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Human serum 97% 79%-117%
Cell culture supernatants 91% 73%-115%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 56.6 4.4 7.8
2 20 85.9 5.7 6.6
3 20 259.5 12.1 4.7

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 58.2 2.8 4.9
2 24 89.1 6.6 7.4
3 24 247.7 16.2 6.5

製品概要
KE00196 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CCL24 ELISA kit is to be used to detect and quantify protein levels of endogenous CCL24. The assay recognizes human CCL24. An antibody specific for CCL24 has been pre-coated onto the microwells. The CCL24 protein in samples is captured by the coated antibody after incubation.Following extensive washing, another antibody of biotinylated specific for CCL24 is added to detect the captured CCL24 protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
C C motif chemokine 24, CCL24, CK beta 6, Ckb 6, Eotaxin 2, MPIF 2, MPIF2, SCYA24, Small inducible cytokine A24
背景説明

CCL24, known as eotaxin-2, is a chemokine that promotes cell trafficking and regulates inflammatory and fibrotic activities mainly through the C-C motif chemokine receptor 3 (CCR3) complex. It is expressed by activated T cells, monocytes, epithelial and endothelial cells as well as by activated fibroblasts. CCL24 is considered a contributor to kinds of atopic disorders, parasitic infections and numerous systemic diseases. CCL24 is also a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line.


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