Human CCL4 ELISA Kit
Cat no : KE00062
Synonyms
ACT2, C C motif chemokine 4, CCL4, chemokine (C C motif) ligand 4, HC21, LAG 1, LAG1, MIP 1 beta, MIP1B, MIP1B1, PAT 744, SCYA2, SCYA4, Small inducible cytokine A4
Validation Data Gallery
Product Information
KE00062 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CCL4 ELISA kit is to be used to detect and quantify protein levels of endogenous CCL4. The assay recognizes human CCL4. A polyclonal antibody specific for CCL4 has been pre-coated onto the microwells. The CCL4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for CCL4 is added to detect the captured CCL4 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
| Product name | Human CCL4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 1 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6351 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatants | 101% | 92%-113% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | ? ug/ml | ? | ?% |
| 2 | 20 | ? ug/ml | ? | ?% |
| 3 | 20 | ? ug/ml | ? | ?% |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | ? ug/ml | ? | ?% |
| 2 | 20 | ? ug/ml | ? | ?% |
| 3 | 20 | ? ug/ml | ? | ?% |
Background Information
CCL4, also known as Macrophage inflammatory protein-1β (MIP-1β), is a CC chemokine with specificity for CCR5 receptors, and is one of the major HIV-suppressive factors produced by CD8+ T-cells. MIP-1β is an acidic protein composed of 69 amino acids that is produced by many cells, particularly macrophages, dendritic cells, and lymphocytes. MIP-1β is responsible for the activation of PMN and is involved in acute neutrophilic inflammation. Recombinant MIP-1β induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ACT2, C C motif chemokine 4, CCL4, chemokine (C C motif) ligand 4, HC21, LAG 1, LAG1, MIP 1 beta, MIP1B, MIP1B1, PAT 744, SCYA2, SCYA4, Small inducible cytokine A4 |
Publications
| Species | Sample Type | Title |
|---|---|---|
Adv Sci (Weinh) S100A5 Attenuates Efficiency of Anti-PD-L1/PD-1 Immunotherapy by Inhibiting CD8+ T Cell-Mediated Anti-Cancer Immunity in Bladder Carcinoma |