Speedy™ Human BTLA/CD272 One-Step ELISA Kit
Sensitivity
0.6 pg/mL
Range
3.13-200 pg/mL
Reactivity
Human
Cat no : SE50067
Synonyms
BTLA, B and T lymphocyte attenuator, B and T lymphocyte associated, B- and T-lymphocyte attenuator, B- and T-lymphocyte-associated protein
Validation Data Gallery
Product Information
| Product name | Speedy™ Human BTLA/CD272 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 0.6 pg/mL |
| Range | 3.13-200 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 151888 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 76% | 74%-80% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 100.6 | 1.6 | 1.6 |
| 2 | 8 | 26.3 | 0.5 | 1.9 |
| 3 | 8 | 13.5 | 0.4 | 3.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 99.7 | 2.0 | 2.0 |
| 2 | 16 | 25.7 | 0.8 | 3.1 |
| 3 | 16 | 13.1 | 0.6 | 4.6 |
Background Information
BTLA, also known as CD272, is a member of the CD28 superfamily and is a type I membrane glycoprotein identified as an inhibitory receptor. BTLA is extensively expressed in lymph nodes, thymus, and spleen, with little or no expression in organs such as the heart, kidney, brain, and liver. Among immune cells, BTLA is primarily expressed in B and T cells, with higher expression in B cells compared to T cells in the mouse spleen. BTLA plays a crucial role in regulating stimulatory and inhibitory signals in immune responses.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | BTLA, B and T lymphocyte attenuator, B and T lymphocyte associated, B- and T-lymphocyte attenuator, B- and T-lymphocyte-associated protein |