Speedy™ Human B7-H3/CD276 One-Step ELISA Kit
Sensitivity
0.2 pg/mL
Range
19.5-1250 pg/mL
Reactivity
Human
Cat no : SE50017
Synonyms
CD276, 4Ig-B7-H3, B7 H3, B7 homolog 3, B7H3
Validation Data Gallery
Product Information
.
| Product name | Speedy™ Human B7-H3/CD276 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell cuture supernatant, Urine, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.2 pg/mL |
| Range | 19.5-1250 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 80381 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 99% | 89%-115% |
| Cell culture supernatant | 110% | 105%-120% |
| Urine | 102% | 93%-115% |
| Cell lysate | 101% | 86%-119% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 709.5 | 14.4 | 2.0 |
| 2 | 8 | 161.6 | 5.2 | 3.2 |
| 3 | 8 | 85.7 | 1.8 | 2.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 728.7 | 28.2 | 3.9 |
| 2 | 16 | 166.7 | 7.9 | 4.7 |
| 3 | 16 | 81.3 | 5.6 | 6.8 |
Background Information
B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor. Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy. Soluble form of B7-H3 (sB7-H3), released by monocytes, dendritic cells, activated T cells, and B7-H3-positive tumor cells, has been detected in human blood samples and cell culture supernatants.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD276, 4Ig-B7-H3, B7 H3, B7 homolog 3, B7H3 |