Speedy™ Human Angiogenin One-Step ELISA Kit
Sensitivity
0.5 pg/mL
Range
3.13-200 pg/mL
Reactivity
Human
Cat no : SE50061
Synonyms
ANG, ANG 1, ANG I, ANG1, ANGI
Validation Data Gallery
Product Information
| Product name | Speedy™ Human Angiogenin One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.5 pg/mL |
| Range | 3.13-200 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 283 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 92% | 77%-97% |
| Cell culture supernatant | 108% | 104%-111% |
| Cell lysate | 80% | 72%-86% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 100.9 | 2.9 | 2.9 |
| 2 | 8 | 26.1 | 0.4 | 1.5 |
| 3 | 8 | 7.4 | 0.2 | 2.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 100.2 | 2.4 | 2.4 |
| 2 | 16 | 25.6 | 0.7 | 2.7 |
| 3 | 16 | 7.2 | 0.3 | 4.2 |
Background Information
Angiogenin (ANG), an angiogenic ribonuclease, is a member of the vertebrate-specific, secreted RNASE superfamily. Angiogenin, originally identified as a tumor angiogenic factor, was related with the growth and metastasis of numerous tumors. Angiogenin has been proposed as a permissive factor for angiogenesis induced by other angiogenic factors, including vascular endothelial growth factor (VEGF), basic fibroblast growth factor, acidic fibroblast growth factor, and epidermal growth factor. Angiogenin production and secretion may be stimulated by hypoxia. Increased angiogenin serum levels have been associated with the incidence and severity of several human tumors, including HCC.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ANG, ANG 1, ANG I, ANG1, ANGI |