Speedy™ Human Alpha-1-Antitrypsin One-Step ELISA Kit
Sensitivity
0.20 ng/mL
Range
0.78-50 ng/mL
Reactivity
Human
Cat no : SE50049
Synonyms
a1-antitrypsin, Serpin A1, SERPINA1, A1A, A-1-Antitrypsin
Validation Data Gallery
Product Information
| Product name | Speedy™ Human Alpha-1-Antitrypsin One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Saliva, Urine, Human milk |
| Assay type | Sandwich |
| Sensitivity | 0.20 ng/mL |
| Range | 0.78-50 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5265 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 102% | 96%-106% |
| Urine | 96% | 85%-101% |
| Saliva | 102% | 88%-114% |
| Human milk | 107% | 91%-116% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 26.60 | 1.13 | 4.25 |
| 2 | 8 | 6.06 | 0.12 | 1.98 |
| 3 | 8 | 2.83 | 0.10 | 3.53 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 26.23 | 0.97 | 3.70 |
| 2 | 16 | 6.07 | 0.26 | 4.28 |
| 3 | 16 | 2.81 | 0.09 | 3.20 |
Background Information
Alpha-1-antitrypsin (AAT) is a protein for the gene called SERPINA1, which is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. AAT is a glycoprotein synthesized primarily by hepatocytes, with smaller amountssynthesized by intestinal epithelial cells, neutrophils, pulmonary alveolar cells and macrophages. AAT is the most abundant, endogenous serine protease inhibitor in blood circulation and it has been implicated in regulating vital fluid phase biological events such as blood coagulation, fibrinolysis, complement activation, apoptosis, reproduction, tumor progression and inflammatory response. The primary function of AAT is thought to be the inactivation of neutrophil elastase and other endogenous serine proteases. Defects in SERPINA1 can cause emphysema or liver disease.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | a1-antitrypsin, Serpin A1, SERPINA1, A1A, A-1-Antitrypsin |