Human APCDD1 ELISA Kit
Sensitivity
0.16 ng/mL
Range
0.78-50 ng/mL
Reactivity
Human
Cat no : KE00915
Synonyms
Adenomatosis polyposis coli down-regulated 1 protein, B7323, DRAPC1, FP7019, Protein APCDD1
Validation Data Gallery
Product Information
KE00915 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human APCDD1 ELISA kit is to be used to detect and quantify protein levels of endogenous human APCDD1. The assay recognizes human APCDD1. An antibody specific for human APCDD1 has been pre-coated onto the microwells. The human APCDD1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human APCDD1 is added to detect the captured human APCDD1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human APCDD1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.16 ng/mL |
| Range | 0.78-50 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 147495 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 85% | 81%-94% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 28.51 | 0.58 | 2.03 |
| 2 | 8 | 6.72 | 0.34 | 5.00 |
| 3 | 8 | 3.51 | 0.17 | 4.97 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 27.51 | 1.41 | 5.14 |
| 2 | 16 | 6.86 | 0.31 | 4.48 |
| 3 | 16 | 3.52 | 0.20 | 5.61 |
Background Information
APCDD1 (Adenomatosis Polyposis Coli Down-Regulated 1) is a membrane-bound glycoprotein and a single-pass transmembrane protein that primarily functions through its large extracellular domain. APCDD1 is an endogenous inhibitor of the Wnt signaling pathway and is expressed in various tissues, including the nervous system, hair follicles, and vascular system. It inhibits Wnt signaling by interacting with Wnt ligands (such as Wnt3a) and a key component of the Wnt signaling pathway, LRP5. Additionally, APCDD1 has a dual inhibitory function, capable of simultaneously inhibiting both the Wnt and BMP signaling pathways. During hair follicle development, the expression of APCDD1 is regulated by Wnt signaling and modulates the hair follicle cycle through a negative feedback mechanism.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Adenomatosis polyposis coli down-regulated 1 protein, B7323, DRAPC1, FP7019, Protein APCDD1 |