Human A1BG ELISA Kit0 Publications

カタログ番号: KE00130

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human A1BG ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatants

アッセイ方法:
Sandwich

感度:
21.5 pg/mL

測定範囲:
125-4000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Human serum 104% 90%-125%
Human plasma 101% 77%-122%
Cell culture supernatants 98% 80%-111%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 257.9 14.7 5.7
2 20 517.3 23.3 4.5
3 20 2,133.3 107.2 5.0

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 266.1 21.2 8.0
2 24 504.5 35.8 7.1
3 24 2,072.8 105.0 5.1

製品概要
KE00130 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The A1BG ELISA kit is to be used to detect and quantify protein levels of endogenous A1BG. The assay recognizes human A1BG. An antibody specific for A1BG has been pre-coated onto the microwells. The A1BG protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for A1BG is added to detect the captured A1BG protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
A1B, A1BG, ABG, alpha 1 B glycoprotein, Alpha 1B glycoprotein, DKFZp686F0970, GAB, HYST2477
背景説明

Alpha-1B-glycoprotein (A1BG) is a plasma protein and is a member of the immunoglobulin superfamily. Alpha-1B-glycoprotein contains 474 amino acids and has 5 intrachain disulfide bonds. It has been reported that cysteine-rich secretory protein 3 (CRISP-3) is a specific and high-affinity ligand of alpha-1B-glycoprotein. Alpha-1B-glycoprotein-CRISP-3 complex displays a similar function in protecting the circulation from a potentially harmful effect of free CRISP3. Alpha-1B-glycoprotein is present in normal adult plasma at an average concentration of 22 mg/dl. Elevated alpha-1B-glycoprotein levels in blood have been associated with several kinds of cancer, including non-small cell lung cancer, endometrial and cervical cancer.


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