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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HuC Polyclonal antibody (55047-1-AP)

    WB analysis using 55047-1-AP (same clone as 55047-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55047-1-AP (HuC antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55047-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Neuro-2a cells using HuC Polyclonal antibody (55047-1-AP)

    IP experiment of Neuro-2a using 55047-1-AP (same clone as 55047-1-PBS)

    IP result of anti-HuC (IP:55047-1-AP, 4ug; Detection:55047-1-AP 1:5000) with Neuro-2a cells lysate 1440 ug. This data was developed using the same antibody clone with 55047-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using HuC Polyclonal antibody (55047-1-AP)

    IHC staining of human brain using 55047-1-AP (same clone as 55047-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55047-1-AP (HuC antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55047-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HuC Polyclonal antibody (55047-1-AP)

    IHC staining of human lung cancer using 55047-1-AP (same clone as 55047-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55047-1-AP (HuC antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55047-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HuC Polyclonal antibody (55047-1-AP)

    IHC staining of human lung cancer using 55047-1-AP (same clone as 55047-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55047-1-AP (HuC antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55047-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using HuC Polyclonal antibody (55047-1-AP)

    IHC staining of human brain using 55047-1-AP (same clone as 55047-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55047-1-AP (HuC antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55047-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

HuC Polyclonal antibody, PBS Only

HuC Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 55047-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

ELAVL 3, ELAVL3, ELAV-like protein 3, Hu antigen C, Hu-antigen C

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55047-1-PBS targets HuC in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C)
Calculated molecular weight 40 kDa
Observed molecular weight 36-38 kDa
GenBank accession numberNM_001420
Gene Symbol HuC
Gene ID (NCBI) 1995
RRIDAB_10859256
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14576
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

HUC , belongs to the RRM elav family. It binds to AU-rich sequences (AREs) of target mRNAs, including VEGF mRNA. ELAVL3 may also bind poly-A tracts via RRM 3. It is involved in neuronal differentiation and maintenance. The antibody has no cross reaction to other ELAVL members.