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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Hsc70 Monoclonal antibody (66442-1-Ig)

    WB analysis using 66442-1-Ig (same clone as 66442-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66442-1-Ig (Hsc70 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66442-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Hsc70 Monoclonal antibody (66442-1-Ig)

    WB analysis of HeLa using 66442-1-Ig (same clone as 66442-1-PBS)

    Western blot analysis of Hsc70 in various cell lines using 66442-1-Ig (Hsc70 Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66442-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Recombinant protein using Hsc70 Monoclonal antibody (66442-1-Ig)

    WB analysis of Recombinant protein using 66442-1-Ig (same clone as 66442-1-PBS)

    6*His tagged-recombinant HSP70 (291-641 aa) and HSC70 (346-646 aa) proteins were subjected to SDS PAGE followed by western blot with 66183-1-Ig (HSP70 antibody), 66442-1-Ig (HSC70 antibody), and 66005-1-Ig (His antibody), respectively, at dilution of 1:50000, incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66442-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Hsc70 Monoclonal antibody (66442-1-Ig)

    WB analysis of HEK-293 using 66442-1-Ig (same clone as 66442-1-PBS)

    WB result of Hsc70 antibody (66442-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Hsc70 transfected HEK-293 cells. This data was developed using the same antibody clone with 66442-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Hsc70 Monoclonal antibody (66442-1-Ig)

    IHC staining of human ovary tumor using 66442-1-Ig (same clone as 66442-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66442-1-Ig (Hsc70 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66442-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Hsc70 Monoclonal antibody (66442-1-Ig)

    IHC staining of human ovary tumor using 66442-1-Ig (same clone as 66442-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66442-1-Ig (Hsc70 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66442-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using Hsc70 Monoclonal antibody (66442-1-Ig)

    IF Staining of HEK-293 using 66442-1-Ig (same clone as 66442-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using Hsc70 antibody (66442-1-Ig, Clone: 1A4B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66442-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Hsc70 Monoclonal antibody (66442-1-Ig)

    IF Staining of HeLa using 66442-1-Ig (same clone as 66442-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Hsc70 antibody (66442-1-Ig, Clone: 1A4B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66442-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Hsc70 Monoclonal antibody, PBS Only

Hsc70 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66442-1-PBS
Clone No.1A4B3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

HSPA8, 1A4B3, EC:3.6.4.10, Heat shock cognate 71 kDa protein, Heat shock protein family A member 8

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66442-1-PBS targets Hsc70 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag17910

Product name: Recombinant human Hsc70 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 288-587 aa of BC007276

Sequence: QKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAVDKSTGKENKITITNDKGRLSKEDIERMVQEAEKYKAEDEKQRDKVSSKNSLESYAFNMKATVEDEKLQGKINDEDKQKILDKCNEIINWLDKNQTAEKEEFEHQQKELEKVCNPIITKLYQSAGGMPGGMPGGFPGGGAPPSGGASSGPTIEEVD

相同性解析による交差性が予測される生物種
Full Name heat shock 70kDa protein 8
Calculated molecular weight 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC007276
Gene Symbol HSC70
Gene ID (NCBI) 3312
RRIDAB_2881812
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP11142
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

HSPA8 (also known as HSC70) is a member of the HSPA (HSP70) family of heat-shock proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSPA8 is a constitutively expressed cytosol/nuclear protein able to translocate between cytoplasm and nucleus. Recently it has been reported that HSPA8 can interact with α-synuclein, the critical pathological protein of Parkinson's disease, indicating its implication in neurodegenerative disease. (PMID: 21832061)