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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Histone H3 Recombinant antibody (81984-2-RR)

    WB analysis using 81984-2-RR (same clone as 81984-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81984-2-RR (Histone H3 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81984-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Histone H3 Recombinant antibody, PBS Only (81984-2-PBS)

    IHC staining of mouse brain using 81984-2-PBS

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81984-2-PBS (Histone H3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Histone H3 Recombinant antibody (81984-2-RR)

    IHC staining of mouse brain using 81984-2-RR (same clone as 81984-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81984-2-RR (Histone H3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81984-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Histone H3 Recombinant antibody (81984-2-RR)

    IHC staining of mouse brain using 81984-2-RR (same clone as 81984-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81984-2-RR (Histone H3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81984-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Histone H3 Recombinant antibody (81984-2-RR)

    IF Staining of HeLa using 81984-2-RR (same clone as 81984-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Histone H3 antibody (81984-2-RR, Clone: 241079B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81984-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using Histone H3 Recombinant antibody (81984-2-RR)

    FC experiment of HepG2 using 81984-2-RR (same clone as 81984-2-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Histone H3 Recombinant antibody (81984-2-RR, Clone:241079B2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 81984-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Histone H3 Recombinant antibody, PBS Only (81984-2-PBS)

    FC experiment of HeLa using 81984-2-PBS

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Histone H3 Recombinant antibody, PBS Only (81984-2-PBS, Clone:241079B2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 81984-2-RR

    Affinity and Kinetic Characterization of 81984-2-RR

    Biolayer interferometry (BLl) kinetic assays of 81984-2-RR against Human Histone H3 were performed. The affinity constant is 12.2 nM.

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Histone H3 Recombinant antibody, PBS Only

Histone H3 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 81984-2-PBS
Clone No.241079B2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

HIST2H3A, 241079B2, H3C13, H3C14, H3C15

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81984-2-PBS targets Histone H3 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Histone H3 fusion protein Ag10644 相同性解析による交差性が予測される生物種
Full Name histone cluster 2, H3a
Calculated molecular weight 136 aa, 15 kDa
Observed molecular weight15-17 kDa
GenBank accession numberBC015544
Gene Symbol Histone H3
Gene ID (NCBI) 333932
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ71DI3
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Histone-H3, histone cluster 2, H3a is the core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machinery which requires DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone-H3 is expressed during S phase; then expression strongly decreases as cell division slows down during the process of differentiation.

Protocols

Product Specific Protocols
IHC protocol for Histone H3 antibody 81984-2-PBSDownload protocol
FC protocol for Histone H3 antibody 81984-2-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols