Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
18064-1-PBS targets Histone H3.4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12671 Product name: Recombinant human HIST3H3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-136 aa of BC101837 Sequence: MARTKQTARKSTGGKAPRKQLATKVARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLMREIAQDFKTDLRFQSSAVMALQEACESYLVGLFEDTNLCVIHAKRVTIMPKDIQLARRIRGERA 相同性解析による交差性が予測される生物種 |
| Full Name | histone cluster 3, H3 |
| Calculated molecular weight | 136 aa, 16 kDa |
| Observed molecular weight | 16 kDa |
| GenBank accession number | BC101837 |
| Gene Symbol | Histone H3.4 |
| Gene ID (NCBI) | 8290 |
| RRID | AB_3669290 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16695 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
HIST3H3, also known as H3.1t and H3.4, is a histone H3 protein variant that bears the H3.1 variant hallmark cysteine 96 but includes three extra amino acid substitutions. H3.1t was found to be expressed in primary human spermatocytes at the pachytene stage, similar to the human testis-specific histone H1t variant, where it appears therefore to take part in the process of spermatogenesis (PMID: 21782046).