Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31048-1-AP targets Histone H2B in WB, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Reactivity | Human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag33975 Product name: Recombinant human HIST1H2BA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-127 aa of BC066242 Sequence: MPEVSSKGATISKKGFKKAVVKTQKKEGKKRKRTRKESYSIYIYKVLKQVHPDTGISSKAMSIMNSFVTDIFERIASEASRLAHYSKRSTISSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 相同性解析による交差性が予測される生物種 |
| Full Name | histone cluster 1, H2ba |
| Observed molecular weight | 15 kDa |
| GenBank accession number | BC066242 |
| Gene Symbol | Histone H2B |
| Gene ID (NCBI) | 255626 |
| RRID | AB_3669832 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96A08 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Histones are the basic nuclear proteins responsible for the nucleosome structure of the chromosomal fiber. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.Histone H2BC1 is testis specific.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Histone H2B antibody 31048-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |