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Validation Data Gallery

  • IP of GFP-His fusion protein from transfected HEK293T cells using His Fab-Trap® Agarose (hfa) followed by a two-step elution with His-Peptide (hp). I: Input, F: Flow-Through, E1: 1st elution, E2: 2nd elution, R: Residual.

    IP of GFP-His fusion protein from transfected HEK293T cells using His Fab-Trap® Agarose (hfa) followed by a two-step elution with His-Peptide (hp). I: Input, F: Flow-Through, E1: 1st elution, E2: 2nd elution, R: Residual.

  • IP of GFP-His fusion protein from transfected HEK293T cells using His Fab-Trap® Magnetic Agarose (hfma) followed by a two-step elution with His-Peptide (hp). I: Input, F: Flow-Through, E1: 1st elution, E2: 2nd elution, R: Residual.

    IP of GFP-His fusion protein from transfected HEK293T cells using His Fab-Trap® Magnetic Agarose (hfma) followed by a two-step elution with His-Peptide (hp). I: Input, F: Flow-Through, E1: 1st elution, E2: 2nd elution, R: Residual.

ChromoTek His-Peptide

HPLC-purified His-peptide
Cat No. hp

Peptide

His Tag

Applications

Elution

Conjugate

Unconjugated

Synonyms

His peptide, HHHHHH, 6*His-Peptide

Size/Concentration: 

-/ -

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Product Information

HPLC-purified His-peptide

DescriptionHPLC-purified His-peptide
ApplicationsElution of proteins fused to His-tag bound to His Fab-Trap®.
SequenceHHHHHH
FormLyophilized Powder
ConjugateUnconjugated
Molecular Weight841 Da
Purity95%
ReconstitutionReconstitute 1 mg His-Peptide (hp-1) in 1190 µl PBS, which results in a final concentration of 1 mM. Vortex for 1 min to dissolve the powder and dilute to 100 µM for the working solution.
Storage ConditionUpon receipt store at -20°C. Aliquot upon reconstitution and store at -20°C. Avoid repeated freeze/thaw cycles.
StabilityStable for 1 year upon receipt
ShipmentShipped at ambient temperature