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Validation Data Gallery

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  • Western Blot (WB) analysis of 4T1 cells using Her2 / ErbB2 Recombinant antibody (84128-5-RR)

    WB analysis of 4T1 using 84128-5-RR

    4T1 cells were subjected to SDS PAGE followed by western blot with 84128-5-RR (Her2 / ErbB2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of 4T1 cells using Her2 / ErbB2 Recombinant antibody (84128-5-RR)

    IF Staining of 4T1 using 84128-5-RR

    Immunofluorescent analysis of (4% PFA) fixed 4T1 cells using Her2 / ErbB2 antibody (84128-5-RR, Clone: 241366F4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84128-5-RR

    Affinity and Kinetic Characterization of 84128-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84128-5-RR against Mouse Her2 / ErbB2 were performed. The affinity constant is 0.356 nM.

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HER2/ErbB2 Recombinant antibody

HER2/ErbB2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84128-5-RR
Clone No.241366F4

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Her2 / ErbB2, Her2, Erbb2, EC:2.7.10.1, CD340

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected in4T1 cells
Positive IF/ICC detected in4T1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84128-5-RR targets HER2/ErbB2 in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
Calculated molecular weight139 kDa
Observed molecular weight185 kDa
GenBank accession numberNM_001003817.1
Gene Symbol Erbb2
Gene ID (NCBI) 13866
RRIDAB_3671687
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP70424
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Her2, or ErbB2, is a member of the EGF receptor family of receptor tyrosine kinases. It has no ligand-binding domain of its own. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signaling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase.

Protocols

Product Specific Protocols
WB protocol for HER2/ErbB2 antibody 84128-5-RRDownload protocol
IF protocol for HER2/ErbB2 antibody 84128-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols