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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Harmonin Polyclonal antibody (11358-1-AP)

    WB analysis using 11358-1-AP (same clone as 11358-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11358-1-AP (Harmonin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11358-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Harmonin Polyclonal antibody (11358-1-AP)

    WB analysis of HEK-293 using 11358-1-AP (same clone as 11358-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11358-1-AP (Harmonin antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11358-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using Harmonin Polyclonal antibody (11358-1-AP)

    IP experiment of HEK-293 using 11358-1-AP (same clone as 11358-1-PBS)

    IP result of anti-Harmonin (IP:11358-1-AP, 4ug; Detection:11358-1-AP 1:600) with HEK-293 cells lysate 3000ug. This data was developed using the same antibody clone with 11358-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of Human Colon cancer tissue using Harmonin Polyclonal antibody (11358-1-AP)

    IHC staining of Human Colon cancer using 11358-1-AP (same clone as 11358-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 11358-1-AP (Harmonin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11358-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of Human Colon cancer tissue using Harmonin Polyclonal antibody (11358-1-AP)

    IHC staining of Human Colon cancer using 11358-1-AP (same clone as 11358-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 11358-1-AP (Harmonin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11358-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of Human Colon cancer tissue using Harmonin Polyclonal antibody (11358-1-AP)

    IHC staining of Human Colon cancer using 11358-1-AP (same clone as 11358-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 11358-1-AP (Harmonin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11358-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of Human Colon cancer tissue using Harmonin Polyclonal antibody (11358-1-AP)

    IHC staining of Human Colon cancer using 11358-1-AP (same clone as 11358-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 11358-1-AP (Harmonin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11358-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of Human Colon cancer tissue using Harmonin Polyclonal antibody (11358-1-AP)

    IHC staining of Human Colon cancer using 11358-1-AP (same clone as 11358-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 11358-1-AP (Harmonin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11358-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Harmonin Polyclonal antibody (11358-1-AP)

    IF Staining of HepG2 using 11358-1-AP (same clone as 11358-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Harmonin antibody (11358-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11358-1-PBS in a different storage buffer formulation.

Harmonin Polyclonal antibody, PBS Only

Harmonin Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11358-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11358-1-PBS targets Harmonin in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1919

Product name: Recombinant human USH1C protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-392 aa of BC016057

Sequence: MDRKVAREFRHKVDFLIENDAEKDYLYDVLRMYHQTMDVAVLVGDLKLVINEPSRLPLFDAIRPLIPLKHQVEYDQLTPRRSRKLKEVRLDRLHPEGLGLSVRGGLEFGCGLFISHLIKGGQADSVGLQVGDEIVRINGYSISSCTHEEVINLIRTKKTVSIKVRHIGLIPVKSSPDEPLTWQYVDQFVSESGGVRGSLGSPGNRENKEKKVFISLVGSRGLGCSISSGPIQKPGIFISHVKPGSLSAEVGLEIGDQIVEVNGVDFSNLDHKEGRELFMTDRERLAEARQRELQRQELLMQKRLAMESNKILQEQQEMERQRRKEIAQKAAEENERYRKEMEQIVEEEEKFKKQWEEDWGSKEQLLLPKTITAEVHPVPLRKPKYDQGVEPE

相同性解析による交差性が予測される生物種
Full Name Usher syndrome 1C (autosomal recessive, severe)
Calculated molecular weight 552 aa, 62 kDa
Observed molecular weight 68-73 kDa
GenBank accession numberBC016057
Gene Symbol USH1C/Harmonin
Gene ID (NCBI) 10083
RRIDAB_2214137
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6N9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Harmonin, also known as USH1C, is a scaffold protein which interacts with other USH1 proteins (USH1B/myosin VIIa and USH1G/sans) to form complex and is implicated in proper development, morphogenesis, and structural integrity of the hair cell mechanoreceptor. Mutations of Harmonin cause Usher syndrome type 1 (congenital deafness and balance disorder, and early-onset sight loss).

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