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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Haptoglobin Recombinant antibody (83702-5-RR)

    WB analysis using 83702-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83702-5-RR (Haptoglobin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Haptoglobin Recombinant antibody (83702-5-RR)

    IHC staining of mouse liver using 83702-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83702-5-RR (Haptoglobin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83702-5-RR

    Affinity and Kinetic Characterization of 83702-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83702-5-RR against Mouse Haptoglobin were performed. The affinity constant is 0.291 nM.

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Haptoglobin Recombinant antibody

Haptoglobin Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83702-5-RR
Clone No.240785A1

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Synonyms

Hp, HP 1, 240785A1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse serum, mouse lung tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83702-5-RR targets Haptoglobin in WB, IHC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name haptoglobin
Calculated molecular weight39kDa
Observed molecular weight 42 kDa
GenBank accession numberNM_017370.2
Gene Symbol Hp
Gene ID (NCBI) 15439
RRIDAB_3671304
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ61646
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Neutrophils contained a large amount of highly glycosylated Hp (β-chain 45-65 kDa) synthesized in neutrophil precursors and stored in specific granules and a small amount of Hp (β-chain 39 kDa) endocytosed from plasma and stored in secretory vesicles (PMID: 16543473).

Protocols

Product Specific Protocols
WB protocol for Haptoglobin antibody 83702-5-RRDownload protocol
IHC protocol for Haptoglobin antibody 83702-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols