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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Haptoglobin Polyclonal antibody (16665-1-AP)

    WB analysis using 16665-1-AP (same clone as 16665-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16665-1-AP (Haptoglobin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16665-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Haptoglobin Polyclonal antibody (16665-1-AP)

    WB analysis using 16665-1-AP (same clone as 16665-1-PBS)

    Human plasma were subjected to SDS PAGE followed by western blot with 16665-1-AP (Haptoglobin antibody) at dilution of 1:18000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16665-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Haptoglobin Polyclonal antibody (16665-1-AP)

    IHC staining of human liver cancer using 16665-1-AP (same clone as 16665-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16665-1-AP (Haptoglobin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16665-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Haptoglobin Polyclonal antibody (16665-1-AP)

    IHC staining of human liver cancer using 16665-1-AP (same clone as 16665-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16665-1-AP (Haptoglobin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16665-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Haptoglobin Polyclonal antibody (16665-1-AP)

    IHC staining of human liver cancer using 16665-1-AP (same clone as 16665-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16665-1-AP (Haptoglobin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16665-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Haptoglobin Polyclonal antibody (16665-1-AP)

    IHC staining of human liver cancer using 16665-1-AP (same clone as 16665-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16665-1-AP (Haptoglobin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16665-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Haptoglobin Polyclonal antibody (16665-1-AP)

    IHC staining of human lung cancer using 16665-1-AP (same clone as 16665-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16665-1-AP (Haptoglobin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16665-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Haptoglobin Polyclonal antibody (16665-1-AP)

    IHC staining of human lung cancer using 16665-1-AP (same clone as 16665-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16665-1-AP (Haptoglobin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16665-1-PBS in a different storage buffer formulation.

Haptoglobin Polyclonal antibody, PBS Only

Haptoglobin Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 16665-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

HP, BP, HAPT, HP2 ALPHA 2, HPA1S

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16665-1-PBS targets Haptoglobin in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag10143

Product name: Recombinant human HP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 179-406 aa of BC058031

Sequence: MVSHHNLTTGATLINEQWLLTTAKNLFLNHSENATAKDIAPTLTLYVGKKQLVEIEKVVLHPNYSQVDIGLIKLKQKVSVNERVMPICLPSKDYAEVGRVGYVSGWGRNANFKFTDHLKYVMLPVADQDQCIRHYEGSTVPEKKTPKSPVGVQPILNEHTFCAGMSKYQEDTCYGDAGSAFAVHDLEEDTWYATGILSFDKSCAVAEYGVYVKVTSIQDWVQKTIAEN

相同性解析による交差性が予測される生物種
Full Name haptoglobin
Calculated molecular weight281aa,31 kDa; 228aa,25 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC058031
Gene Symbol HP
Gene ID (NCBI) 3240
RRIDAB_1939524
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP00738
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

HP(Haptoglobin) is also named as zonulin and belongs to the peptidase S1 family. HP, a plasma glycoprotein that binds free hemoglobin, has a tetrameric structure of 2 alpha(16 kDa and 9 kDa) and 2 beta(40 kDa) polypeptides that are covalently associated by disulfide bonds. In most species, apart from ruminants, Hp has a molecular mass of 100 kDa, consisting of two subunits of 40 kDa and two subunits of 9 kDa, although in a few species, such as man, genetic variant of Hp forms polymers of higher mass(PMID:2361363). Recent studies of haptoglobin show that certain oligosaccharide structures predominate in different diseases. For example, a highly-fucosylated structure is found in breast cancer and ovarian cancer, highly-sialylated structures in Crohn's disease and highly branched structures in alcoholic liver disease and fucosylated haptoglobin is a good serum marker for pancreatic cancer.(PMID:16385567).

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