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Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using HVCN1 Polyclonal antibody (14162-1-AP)

    WB analysis of Raji using 14162-1-AP (same clone as 14162-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 14162-1-AP (HVCN1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14162-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using HVCN1 Polyclonal antibody (14162-1-AP)

    WB analysis of Raji using 14162-1-AP (same clone as 14162-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 14162-1-AP (HVCN1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14162-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using HVCN1 Polyclonal antibody (14162-1-AP)

    WB analysis of PC-3 using 14162-1-AP (same clone as 14162-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 14162-1-AP (HVCN1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14162-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of PC-3 cells using HVCN1 Polyclonal antibody (14162-1-AP)

    IP experiment of PC-3 using 14162-1-AP (same clone as 14162-1-PBS)

    IP result of anti-HVCN1 (IP:14162-1-AP, 4ug; Detection:14162-1-AP 1:300) with PC-3 cells lysate 4000 ug. This data was developed using the same antibody clone with 14162-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HVCN1 Polyclonal antibody (14162-1-AP)

    IHC staining of human tonsillitis using 14162-1-AP (same clone as 14162-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14162-1-AP (HVCN1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14162-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HVCN1 Polyclonal antibody (14162-1-AP)

    IHC staining of human tonsillitis using 14162-1-AP (same clone as 14162-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14162-1-AP (HVCN1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 14162-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HVCN1 Polyclonal antibody (14162-1-AP)

    IHC staining of human tonsillitis using 14162-1-AP (same clone as 14162-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14162-1-AP (HVCN1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 14162-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using HVCN1 Polyclonal antibody (14162-1-AP)

    IF Staining of PC-3 using 14162-1-AP (same clone as 14162-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 14162-1-AP (HVCN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14162-1-PBS in a different storage buffer formulation.

HVCN1 Polyclonal antibody, PBS Only

HVCN1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14162-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

VSOP, Voltage-gated hydrogen channel 1, Voltage sensor domain-only protein, UNQ578/PRO1140, Hydrogen voltage-gated channel 1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14162-1-PBS targets HVCN1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5350

Product name: Recombinant human HVCN1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-103 aa of BC032672

Sequence: MATWDEKAVTRRAKVAPAERMSKFLRHFTVVGDDYHAWNINYKKWENEEEEEEEEQPPPTPVSGEEGRAAAPDVAPAPGPAPRAPLDFRGMLRKLFSSHRFQV

相同性解析による交差性が予測される生物種
Full Name hydrogen voltage-gated channel 1
Calculated molecular weight 273 aa, 32 kDa
Observed molecular weight 28-32 kDa, ~60 kDa
GenBank accession numberBC032672
Gene Symbol HVCN1
Gene ID (NCBI) 84329
RRIDAB_2878023
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96D96
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

HVCN1, also named as VSOP and HV1, Belongs to the hydrogen channel family. HVCN1 mediates the voltage-dependent proton permeability of excitable membranes. It forms a proton-selective channel through which protons may pass in accordance with their electrochemical gradient. Proton efflux, HVCN1 is accompanied by membrane depolarization, facilitates acute production of reactive oxygen species in phagocytosis. HVCN1, the voltage-sensitive proton channel, is present in human sperm and is an important regulator of the functional maturation of sperm. HVCN1 has four isoforms with MW 28-32 kDa or 40 kDa (modification). It has a dimer form with MW ~60 kDa.

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