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Validation Data Gallery

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  • Western Blot (WB) analysis of RAW264.7 using HUS1B Polyclonal antibody (18229-1-AP)

    WB analysis of RAW264.7 using 18229-1-AP (same clone as 18229-1-PBS)

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 18229-1-AP (HUS1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18229-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using HUS1B Polyclonal antibody (18229-1-AP)

    WB analysis of PC-3 using 18229-1-AP (same clone as 18229-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 18229-1-AP (HUS1B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18229-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human stomach tissue using HUS1B Polyclonal antibody (18229-1-AP)

    WB analysis of human stomach using 18229-1-AP (same clone as 18229-1-PBS)

    human stomach tissue were subjected to SDS PAGE followed by western blot with 18229-1-AP (HUS1B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18229-1-PBS in a different storage buffer formulation.

HUS1B Polyclonal antibody, PBS Only

HUS1B Polyclonal Antibody for WB, Indirect ELISA
Cat No. 18229-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18229-1-PBS targets HUS1B in WB, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12712

Product name: Recombinant human HUS1B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-278 aa of BC101697

Sequence: MKFRAKITGKGCLELFIHVSGTVARLAKVCVLRVRPDSLCFGPAGSGGLHEARLWCEVRQGAFQQFRMEGVSEDLDEIHLELTAEHLSRAARSAAGASSLKLQLTHKRRPSLTVAVELVSSLGRARSVVHDLPVRVLPRRVWRDCLPPSLRASDASIRLPRWRTLRSIVERMANVGSHVLVEANLSGRMTLSIETEVVSIQSYFKNLGNPPQSAVGVPENRDLESMVQVRVDNRKLLQFLEGQQIHPTTALCNIWDNTLLQLVLVQEYVSLQYFIPAL

相同性解析による交差性が予測される生物種
Full Name HUS1 checkpoint homolog b (S. pombe)
Calculated molecular weight 278 aa, 31 kDa
Observed molecular weight 31-35 kDa
GenBank accession numberBC101697
Gene Symbol HUS1B
Gene ID (NCBI) 135458
RRIDAB_10694671
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NHY5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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