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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HTRA2 Recombinant monoclonal antibody (83492-7-RR)

    WB analysis using 83492-7-RR (same clone as 83492-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83492-7-RR (HTRA2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83492-7-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HTRA2 Recombinant monoclonal antibody (83492-7-RR)

    IF Staining of HepG2 using 83492-7-RR (same clone as 83492-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HTRA2 antibody (83492-7-RR, Clone: 240496D1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83492-7-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using HTRA2 Recombinant monoclonal antibody (83492-7-RR)

    FC experiment of HeLa using 83492-7-RR (same clone as 83492-7-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HTRA2 Recombinant antibody (83492-7-RR, Clone:240496D1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83492-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83492-7-RR

    Affinity and Kinetic Characterization of 83492-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83492-7-RR against Human CD100 were performed. The affinity constant is 0.574 nM.

HTRA2 Recombinant monoclonal antibody, PBS Only

HTRA2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83492-7-PBS
Clone No.240496D1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

240496D1, EC:3.4.21.108, HtrA serine peptidase 2, OMI, Omi stress-regulated endoprotease

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83492-7-PBS targets HTRA2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag8455

Product name: Recombinant human HTRA2 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 133-458 aa of BC000096

Sequence: AAVPSPPPASPRSQYNFIADVVEKTAPAVVYIEILDRHPFLGREVPISNGSGFVVAADGLIVTNAHVVADRRRVRVRLLSGDTYEAVVTAVDPVADIATLRIQTKEPLPTLPLGRSADVRQGEFVVAMGSPFALQNTITSGIVSSAQRPARDLGLPQTNVEYIQTDAAIDFGNSGGPLVNLDGEVIGVNTMKVTAGISFAIPSDRLREFLHRGEKKNSSSGISGSQRRYIGVMMLTLSPSILAELQLREPSFPDVQHGVLIHKVILGSPAHRAGLRPGDVILAIGEQMVQNAEDVYEAVRTQSQLAVQIRRGRETLTLYVTPEVTE

相同性解析による交差性が予測される生物種
Full Name HtrA serine peptidase 2
Calculated molecular weight 49 kDa
GenBank accession numberBC000096
Gene Symbol HTRA2
Gene ID (NCBI) 27429
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDO43464
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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